tailieunhanh - Báo cáo y học: "The triterpenoid CDDO inhibits expression of matrix metalloproteinase-1, matrix metalloproteinase-13 and Bcl-3 in primary human chondrocytes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: The triterpenoid CDDO inhibits expression of matrix metalloproteinase-1, matrix metalloproteinase-13 and Bcl-3 in primary human chondrocytes. | Available online http content 5 5 R285 Research article Open Access The triterpenoid CDDO inhibits expression of matrix metalloproteinase-1 matrix metalloproteinase-13 and Bcl-3 in primary human chondrocytes Sarah Elliott1 Ezra Hays1 Michael Mayor2 Michael Sporn3 and Matthew Vincenti1 4 1 Department of Medicine Dartmouth Medical School Hanover New Hampshire USA 2Department of Orthopedic Surgery Dartmouth Hitchcock Memorial Hospital Lebanon New Hampshire USA 3Department of Pharmacology and Toxicology Dartmouth Medical School Hanover New Hampshire USA 4VA Hospital White River Junction Vermont USA Corresponding author Matthew Vincenti e-mail Received 11 Apr 2003 Accepted 16 Jun 2003 Published 8 Jul 2003 Arthritis Res Ther 2003 5 R285-R291 DOI ar792 2003 Elliott et al. licensee BioMed Central Ltd Print ISSN 1478-6354 Online ISSN 1478-6362 . This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract A synthetic triterpenoid 2-eyano-3 12-dioxoolean-1 9-dien-28-oie acid CDDO has been reported to have anti-inflammatory properties and to decrease the interleukin-1 IL-1 -induced expression of matrix metalloproteinase-1 MMP-1 and MMP-13. We have shown previously that IL-1 induces expression of the inhibitor of NF-kB IkB family member Bcl-3 and that this contributes to MMP-1 expression. To quantify the effects of CDDO on IL-1-induced MMP-1 MMP-13 and Bcl-3 expression we stimulated the chondrosarcoma cell line SW-1353 and human primary chondrocytes with IL-1 in the presence or absence of CDDO. Harvested RNA was subjected to quantitative real-time reverse-transcriptase polymerase chain reaction. In SW-1353 cells 300 nM CDDO significantly decreased the induction of MMP-1 and MMP-13 by IL-1. In human primary chondrocytes 300 nM CDDO inhibited the induction of these genes