tailieunhanh - Báo cáo y học: "Extracellular mitochondrial DNA and oxidatively damaged DNA in synovial fluid of patients with rheumatoid arthritis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Extracellular mitochondrial DNA and oxidatively damaged DNA in synovial fluid of patients with rheumatoid arthritis. | Arthritis Research Therapy Vol 5 No 5 Hajizadeh et al. Research article Open Access Extracellular mitochondrial DNA and oxidatively damaged DNA in synovial fluid of patients with rheumatoid arthritis Shahin Hajizadeh1 Jeroen DeGroot2 Johan M TeKoppele2 Andrej Tarkowski1 and L Vincent Collins1 1 Department of Rheumatology and Inflammation Research University of Goteborg Goteborg Sweden 2TNO Prevention and Health Gaubius Laboratory Leiden The Netherlands Corresponding author Shahin Hajizadeh e-mail shahin@ Received 21 Mar 2003 Revisions requested 29 Apr 2003 Revisions received 15 May 2003 Accepted 27 May 2003 Published 25 Jun 2003 Arthritis Res Ther 2003 5 R234-R240 DOI ar787 2003 Hajizadeh et al. licensee BioMed Central Ltd Print ISSN 1478-6354 Online ISSN 1478-6362 . This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract We investigated whether plasma and synovial fluid SF samples from patients with rheumatoid arthritis RA contained extracellular mitochondrial DNA mtDNA or the oxidatively damaged DNA adduct 8-hydroxy-2 -deoxyguanosine 8-oxodG . Moreover we correlated the laboratory findings of the patients with RA with their levels of mtDNA and 8-oxodG. SF and plasma samples from 54 patients with RA SF from 30 non-arthritic control subjects and plasma from 22 healthy volunteers were collected. The samples were subjected to polymerase chain reaction PCR using mitochondrial genomic primers and the products were analyzed by SDS-polyacrylamide-gel electrophoresis. The intensities of the PCR-amplified bands were quantified and normalized to a reference sample. Furthermore the SF samples were assayed by enzyme-linked immunosorbent assay for 8-oxodG. Extracellular PCR-amplifiable mtDNA was detected in the SF of 38 of 54 70 patients with RA but not in any of the SF controls. PCR-amplifiable mtDNA was .