tailieunhanh - Báo cáo y học: "Efficacy of treatment with glycosaminoglycans on experimental collagen-induced arthritis in rats"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Efficacy of treatment with glycosaminoglycans on experimental collagen-induced arthritis in rats. | Arthritis Research Therapy Vol 5 No 3 Campo et al. Research article Open Access Efficacy of treatment with glycosaminoglycans on experimental collagen-induced arthritis in rats Giuseppe M Campo1 Angela Avenoso1 Salvatore Campo1 Alida M Ferlazzo2 Domenica Altavilla3 and Alberto Calatroni1 1 Department of Biochemical Physiological and Nutritional Sciences School of Medicine University of Messina Messina Italy 2Department of Morphology Biochemistry Physiology and Animal Production School of Veterinary Medicine University of Messina Messina Italy 3Department of Experimental and Clinical Medicine and Pharmacology School of Medicine University of Messina Messina Italy Corresponding author Giuseppe M Campo e-mail gcampo@ Received 14 Aug 2002 Revisions requested 10 Sep 2002 Revisions received 20 Dec 2002 Accepted 12 Feb 2003 Published 6 Mar 2003 Arthritis Res Ther 2003 5 R122-R131 DOI ar748 2003 Campo et al. licensee BioMed Central Ltd Print ISSN 1478-6354 Online ISSN 1478-6362 . This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract To evaluate the antioxidant activity of the glycosaminoglycans hyaluronic acid HYA and chondroitin-4-sulphate C4S we used a rat model of collagen-induced arthritis CIA . Arthritis was induced in Lewis rats by multiple intradermal injections of 250ựl of emulsion containing bovine type II collagen in complete Freund s adjuvant at the base of the tail and into three to five other sites on the back. Rats were challenged again with the same antigen preparation 7 days later. Disease developed about 11 days after the second immunization. The effects of treatment in the rats were monitored by biochemical parameters and by macroscopic and histological evaluations in blood synovial tissue and articular cartilage. Arthritis produced the following symptoms severe periarticular erythema .