tailieunhanh - Báo cáo y học: "Adverse effects of adenovirus-mediated gene transfer of human transforming growth factor beta 1 into rabbit knees"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Adverse effects of adenovirus-mediated gene transfer of human transforming growth factor beta 1 into rabbit knees. | Arthritis Research Therapy Vol 5 No 3 Mi et al. Research article Open Access Adverse effects of adenovirus-mediated gene transfer of human transforming growth factor beta 1 into rabbit knees Zhibao Mi1 Steven C Ghivizzani1 3 Eric Lechman1 Joseph C Glorioso1 Christopher H Evans2 3 and Paul D Robbins1 Department of Molecular Genetics and Biochemistry University of Pittsburgh School of Medicine Pittsburgh Pennsylvania USA 2Department of Orthopaedic Surgery University of Pittsburgh School of Medicine Pittsburgh Pennsylvania USA 3Present address Center for Molecular Orthopaedics Harvard Medical School Boston Massacuhsetts USA Corresponding author Paul D Robbins e-mail probb@ Received 18 Mar 2002 Revisions requested 8 May 2002 Revisions received 20 Dec 2002 Accepted 4 Feb 2003 Published 1 2 Mar 2003 Arthritis Res Ther 2003 5 R132-R139 DOI ar745 2003 Mi et al. licensee BioMed Central Ltd Print ISSN 1478-6354 Online ISSN 1478-6362 . This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract To examine the effect of transforming growth factor TGF -P1 on the regulation of cartilage synthesis and other articular pathologies we used adenovirus-mediated intra-articular gene transfer of TGF-P1 to both naive and arthritic rabbit knee joints. Increasing doses of adenoviral vector expressing TGF-P1 were injected into normal and antigen-induced arthritis rabbit knee joints through the patellar tendon with the same doses of an adenoviral vector expressing luciferase injected into the contralateral knees as the control. Intra-articular injection of adenoviral vector expressing TGF-P1 into the rabbit knee resulted in dose-dependent TGF-P1 expression in the synovial fluid. Intra-articular TGF-P1 expression in both naive and Keywords arthritis gene therapy cartilage degradation inflammatory nitric oxide rabbit model .