tailieunhanh - Báo cáo y học: "The VH gene repertoire of splenic B cells and somatic hypermutation in systemic lupus erythematosus"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: The VH gene repertoire of splenic B cells and somatic hypermutation in systemic lupus erythematosus. | Arthritis Research Therapy Vol 5 No 2 Fraser et al. Research article The VH gene repertoire of splenic B cells and somatic hypermutation in systemic lupus erythematosus Nicola L W Fraser1 Gary Rowley1 Max Field2 and David I Stott1 Open Access Division of Immunology Infection and Inflammation University of Glasgow Western Infirmary Glasgow Scotland UK 2Department of Rheumatic Diseases Glasgow Royal Infirmary Glasgow Scotland UK Corresponding author Nicola Fraser e-mail nw21y@ Received 27 September 2002 Revisions received 18 December 2002 Accepted 5 January 2003 Published 3 February 2003 Arthritis Res Ther 2003 5 R114-R121 DOI ar627 2003 Fraser et al. licensee BioMed Central Ltd Print ISSN 1478-6354 Online ISSN 1478-6362 . This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any non-commercial purpose provided this notice is preserved along with the article s original URL. Abstract In systemic lupus erythematosus SLE it has been hypothesized that self-reactive B cells arise from virgin B cells that express low-affinity nonpathogenic germline V genes that are cross-reactive for self and microbial antigens which convert to high-affinity autoantibodies via somatic hypermutation. The aim of the present study was to determine whether the VH family repertoire and pattern of somatic hypermutation in germinal centre GC B cells deviates from normal in SLE. Rearranged immunoglobulin VH genes were cloned and sequenced from GCs of a SLE patient s spleen. From these data the GC V gene repertoire and the pattern of somatic mutation during the proliferation of B-cell clones were determined. The results highlighted a bias in VH5 gene family usage previously unreported in SLE and under-representation of the VH1 family which is expressed in 20-30 of IgM B cells of healthy adults and confirmed a defect in negative selection. This is the first study of the splenic GC response in human SLE. Keywords .