tailieunhanh - Báo cáo khoa học: "The response of dehydrated Douglas fir (Pseudotsuga menziesii) pollen to three in vitro viability assays and their relationship to actual fertility"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: "The response of dehydrated Douglas fir (Pseudotsuga menziesii) pollen to three in vitro viability assays and their relationship to actual fertility. | Ann Sà For 1993 50 1-22 Elsevier INRA 1 Original article The response of dehydrated Douglas fir Pseudotsuga menziesii pollen to three in vitro viability assays and their relationship to actual fertility JE Webber 1 M Bonnet-Masimbert2 1 British Columbia Ministry of Forests Research Branch Laboratory 1320 Glyn Road Victoria BC V8W 3E7 Canada 2INRA Station d Amelioration des Arbres Forestiers Centre de Recherche d Orieans Ardon 45160 Olivet France Received 16 March 1992 accepted 30 September 1992 Summary In vitro viability response of Douglas fir pollen stored for various periods 1 to several years was related to actual seed set. Three assay types that provided useful relationships to seed set were respiration RESP percent leachate conductivity COND and percent germination CLASS 1 2 . Before developing the relationship to seed set media effects on germination leaching time for conductivity and pollen hydration effects for all assays were studied. Both simple linear and non-linear regression analyses were compared to percent filled seed per cone FSPC as determined from controlled crossing pollinations. Media type had a significant effect on germination response which in the time of the test 48 h appeared to be related to osmotic rather than metabolic effects. Hydrating stored dehydrated pollen for 16 h at 100 RH and 25 C prior to the analysis had a significant effect on improving the response for conductivity and germination but had no significant effect on respiration. Hydration effects were also apparent on the correlation coefficient r using simple linear regression. For unhydrated and hydrated pollen the r values for assay response and FSPC were and for RESP and for COND and and for CLASS 1 2 germination respectively. Using non-linear regression models the coefficient of determination r2 values for assay response of unhydrated and hydrated pollen against FSPC were and for RESP and and for COND and and .