tailieunhanh - Báo cáo khoa học: "Two-dimensional gel electrophoresis of membrane proteins from ectomycorrhizal fungi"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài:"Two-dimensional gel electrophoresis of membrane proteins from ectomycorrhizal fungi. | Ann Sci For 1992 49 309-319 Elsevier INRA 309 Original article Two-dimensional gel electrophoresis of membrane proteins from ectomycorrhizal fungi B Henrion F Martin INRA Centre de Recherches Forestieres de Nancy Laboratoire de Microbiologie Forestiere Champenoux 54280 Seichamps France Received 12 September 1991 accepted 10 April 1992 Summary A membrane fraction was isolated from the ectomycorrhizal fungi Pisolithus tinctorius and Cenococcum geophilum and from eucalyptus ectomycorrhizas using differential centrifugation. This fraction contained microsomes free of mitochondrial or nuclear membranes and enriched in endoplasmic reticulum Golgi tonoplast and plasma membranes as determined from an analysis of marker enzymes and electron microscopy observations. Four methods of membrane protein solubilisation were assessed on silver-stained 2-dimensional polyacrylamide gels. Gels with limited background staining and streaking and with clearly resolved polypeptides were obtained when p tinctori-us and mycorrhizal proteins were extracted with 2 sodium dodecyl sulphate followed by acetone precipitation. On the other hand the O Farrell buffer containing urea and Nonidet P-40 was selected for solubilisation of c geophilum membrane proteins. An optimization of solubilisation procedures is therefore required for each fungal species. The procedures described make possible the resolution required for meaningful qualitative and quantitative electrophoretic analysis of membrane proteins from ectomycorrhizal fungi and mycorrhizas. Cenococcum geophilum Eucalyptus globulus I Pisolithus tinctorius I ectomycorrhiza electrophoresis I membrane protein I symbiosis-related protein Resume Analyse électrophorétique bidimensionnelle des protéines membranaires de champignons ectomycorhiziens. La differentiation des ectomycorhizes induit de profondes modifications dans la biosynthèse des protéines des partenaires de I assotiation symbiotique. Les structures membranaires de interface symbiotique