tailieunhanh - METHODS IN ENZYMOLOGY

RNA turnover and processing have now been demonstrated to be important steps that directly affect protein synthesis and the cell’s ability to survive in nature. However, the analysis of mRNA decay and polyadenylation in Escherichia coli has long been considered technically difficult. The development over the past 15 years of methods for the isolation and characterization of both mRNA and polyadenylated species has made the study of these important pathways of RNA metabolism more straightforward (Arraiano et al., 1988; O’Hara et al., 1995; Mohanty and Kushner, 1999) | Methods in ENZYMOLOGY Volume 447 RN Turnover in Prokaryotes. Arrhae and Organelles Lynne E. Maquul METHODS IN ENZYMOLOGY Editors-in-Chief JOHN N. ABELSON AND MELVIN I. SIMON Division of Biology California Institute of Technology Pasadena California USA Founding Editors SIDNEY P. COLOWICK AND NATHAN O. KAPLAN Academic Press is an imprint of Elsevier 525 B Street Suite 1900 San Diego California 92101-4495 USA 84 Theobald s Road London wC1X 8RR UK Copyright 2008 Elsevier Inc. All Rights Reserved. No part of this publication may be reproduced or transmitted in any form or by any means electronic or mechanical including photocopy recording or any information storage and retrieval system without permission in writing from the Publisher. The appearance of the code at the bottom of the first page of a chapter in this book indicates the Publisher s consent that copies of the chapter may be made for personal or internal use of specific clients. This consent is given on the condition however that the copier pay the stated per copy fee through the Copyright Clearance Center Inc. for copying beyond that permitted by Sections 107 or 108 of the . Copyright Law. This consent does not extend to other kinds of copying such as copying for general distribution for advertising or promotional purposes for creating new collective works or for resale. Copy fees for pre-2008 chapters are as shown on the title pages. If no fee code appears on the title page the copy fee is the same as for current chapters. 0076-6879 2008 Permissions may be sought directly from Elsevier s Science Technology Rights Department in Oxford UK phone 44 1865 843830 fax 44 1865 853333 E-mail permissions@elsevier. com. You may also complete your request on-line via the Elsevier homepage http by selecting Support Contact then Copyright and Permission and then Obtaining Permissions. For information on all ElsevierAcademic Press publications visit our Web site at .