tailieunhanh - Báo cáo khoa học: Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide

Regulators of G-protein signalling accelerate the GTPase activity of Ga subunits, driving G proteins in their inactive GDP-bound form. This property defines them as GTPase activating proteins. | ỊFEBS Journal Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide Sabine Riekenberg1 Katja Farhat1 Jennifer Debarry2 Holger Heine2 Gunther Jung3 Karl-Heinz Wiesmuller4 and Artur J. Ulmer1 1 Cellular Immunology Department of Immunology and CellBiology Research Center Borstel Germany 2 Innate Immunity Department of Immunology and CellBiology Research Center Borstel Germany 3 Institute of Organic Chemistry University of Tuebingen Germany 4 EMC microcollections GmbH Tuebingen Germany Keywords gene expression lipopeptides macrophages regulator of G-protein signalling Toll-like receptors Correspondence A. J. Ulmer Cellular Immunology and Cell Biology Research Center Borstel Parkallee 22 23845 Borstel Germany Fax 49 4537 188435 Tel 49 4537 188448 E-mail ajulmer@ Received 26 August 2008 revised 12 November 2008 accepted 20 November 2008 doi Regulators of G-protein signalling accelerate the GTPase activity of Ga subunits driving G proteins in their inactive GDP-bound form. This property defines them as GTPase activating proteins. Here the effect of different Toll-like receptor agonists on RGS1 and RGS2 expression in murine bone marrow-derived macrophages and J774 cells was analysed. After stimulation with TLR2 1 or TLR2 6 lipopeptide ligands and the TLR4 MD2 ligand lipopolysaccharide microarray analyses show only modulation of RGS1 and RGS2 among all the regulators of G-protein signalling tested. Real-time PCR confirmed modulation of RGS1 and RGS2. In contrast to RGS2 which was always downregulated RGS1 mRNA was upregulated during the first 30 min after stimulation followed by downregulation. Similar results were also found in the murine macrophage cell line J774. The ligand for intracellular TLR9 modulates RGS1 and RGS2 in a similar manner. However the TLR3 ligand poly I C permanently upregu-lates RGS1 and RGS2 expression indicating a different modulation by the MyD88- and .

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