tailieunhanh - TELEMEDICINE TECHNIQUES AND APPLICATIONS

Two cell lines were created; Flp293A and 293 FLEX, both derived from 293 cells. The former pseudotyped with amphotropic and the latter with GaLV envelopes. Recently, a PG13-based murine producer cell line was also established using this strategy (Loew et al. 2009). A favorable chromosomal site for stable and high retroviral vector production is first identified and tagged. Due to the presence of two heterologous non-compatible FRT sites flanking the tagged retroviral genome, the subsequent re-use of this defined chromosomal site by means of RMCE is than performed to express a therapeutic gene. In order to select cell clones that underwent correct targeted integration reaction, the targeting. | TELEMEDICINE TECHNIQUES AND APPLICATIONS Edited by Georgi Graschew and Stefan Rakowsky Telemedicine Techniques and Applications Edited by Georgi Graschew and Stefan Rakowsky Published by InTech Janeza Trdine 9 51000 Rijeka Croatia Copyright 2011 InTech All chapters are Open Access articles distributed under the Creative Commons Non Commercial Share Alike Attribution license which permits to copy distribute transmit and adapt the work in any medium so long as the original work is properly cited. After this work has been published by InTech authors have the right to republish it in whole or part in any publication of which they are the author and to make other personal use of the work. Any republication referencing or personal use of the work must explicitly identify the original source. Statements and opinions expressed in the chapters are these of the individual contributors and not necessarily those of the editors or publisher. No responsibility is accepted for the accuracy of information contained in the published articles. The publisher assumes no responsibility for any damage or injury to persons or property arising out of the use of any materials instructions methods or ideas contained in the book. Publishing Process Manager Ana Pantar Technical Editor Teodora Smiljanic Cover Designer Jan Hyrat Image Copyright 26kot 2010. Used under license from First published July 2011 Printed in Croatia A free online edition of this book is available at Additional hard copies can be obtained from orders@ Telemedicine Techniques and Applications Edited by Georgi Graschew and Stefan Rakowsky p. cm. ISBN 978-953-307-354-5 ikrrr iJ OPEN ACCESS INI Cvn PUBLISHER INTECH open free online editions of InTech Books and Journals can be found at .