tailieunhanh - Báo cáo khoa học: Light-induced gene expression of fructose 1,6-bisphosphate aldolase during heterotrophic growth in a cyanobacterium, Synechocystis sp. PCC 6803

Synechocystissp. PCC 6803 exhibits light-activated heterotrophic growth (LAHG) under dark conditions with glucose as a carbon source. The light activation is remarkable at a late period of photoautotrophic preculture, such as the late-linear and stationary growth phases. | ỊFEBS Journal Light-induced gene expression of fructose 1 6-bisphosphate aldolase during heterotrophic growth in a cyanobacterium Synechocystis sp. PCC 6803 Yosuke Tabei Katsuhiko Okada Nobuaki Makita and Mikio Tsuzuki Schoolof Life Sciences Tokyo University of Pharmacy and Life Sciences Japan Keywords cyanobacteria glucose degradation light signal transduction Synechocystis Correspondence K. Okada Schoolof Life Sciences Tokyo University of Pharmacy and Life Sciences Horinouchi Hachioji Tokyo 192-0392 Japan Fax 81 42 676 6721 Tel 81 42 676 6716 E-mail kokada@ Received 8 August 2008 revised 18 October 2008 accepted 30 October 2008 doi Synechocystis sp. PCC 6803 exhibits light-activated heterotrophic growth LAHG under dark conditions with glucose as a carbon source. The light activation is remarkable at a late period of photoautotrophic preculture such as the late-linear and stationary growth phases. To understand the physiological effects of light irradiation and glucose under LAHG conditions their effects on the expression of soluble proteins were analyzed by means of 2D-PAGE. Various soluble proteins which were minimal under photoautotrophic preculture conditions were observed clearly under LAHG conditions suggesting that proteins were synthesized actively under these conditions. Fructose 1 6-bisphosphate aldolase one of the glycolytic enzymes was found to be induced under LAHG conditions on 2D-PAGE. The activity of fructose 1 6-bisphosphate aldolase which had decreased during photoautotrophic preculture also increased under LAHG conditions similar to the mRNA level of the encoding gene fbaA. In addition we found that a deletion mutant of sll1330 a putative gene containing a helix-turn-helix DNA-binding motif could not grow under LAHG conditions whereas it could grow photoautotrophically. The increases in the protein level of FbaA and fbaA gene expression observed in wild-type cells under LAHG conditions were greatly

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