tailieunhanh - báo cáo hóa học:" Frequency of Drug-Resistant Variants of HIV-1 Coexistent With Wild-Type in Treatment-Naive Patients of India"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Frequency of Drug-Resistant Variants of HIV-1 Coexistent With Wild-Type in Treatment-Naive Patients of India | Journal of the International AIDS Society BioMed entrai Research article Frequency of Drug-Resistant Variants of HIV-1 Coexistent With Wild-Type in Treatment-Naive Patients of India Naresh Sachdeva1 Shobha Sehgal2 and Sunil K Arora 3 Address Department of Immunopathology Post Graduate Institute of Medical Education and Research Chandigarh India 2Emeritus Professor Department of Immunopathology Post Graduate Institute of Medical Education and Research Chandigarh India and 3Associate Professor Department of Immunopathology Post Graduate Institute of Medical Education and Research Chandigarh India Email Sunil K Arora - skarora_in@ Corresponding author Published 27 July 2005 Journal of the International AIDS Society 2005 7 68 This article is available from http content 7 3 68 Open Access Abstract Context Over the past few years reports of emergence and transmission of drug-resistant strains of HIV have increased especially in western countries. In the context of increased widespread use of zidovudine- and lamivudine-based combinations in India coupled with the genetic diversity of HIV it is essential to generate preliminary data on the frequency of zidovudine- and lamivudine-resistant variants of HIV-1 in North India. Objectives In the present study the authors screened for mutations in the pol gene of HIV-1 associated with resistance to zidovudine and lamivudine in HIV-infected treatment-naive patients from North India. Design and Patients The mutations were screened at codons 70 and 215 conferring resistance to zidovudine and at codon 184 conferring resistance to lamivudine by using a nested amplification refractory mutation system-polymerase chain reaction ARMS-PCR approach from the proviral DNA of 60 patients. Results Most of the patients showed a mixture of both wild-type and mutant virus. In all but 1 patient wild-type virus was observed with respect to each codon. Mutant variants were also observed in many patients especially at codon