tailieunhanh - báo cáo hóa học:" Metformin therapy in a hyperandrogenic anovulatory mutant murine model with polycystic ovarian syndrome characteristics improves oocyte maturity during superovulation"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Metformin therapy in a hyperandrogenic anovulatory mutant murine model with polycystic ovarian syndrome characteristics improves oocyte maturity during superovulation | Sabatini et al. Journal of Ovarian Research 2011 4 8 http content 4 1 8 RESEARCH JOURNAL OF OVARIAN RESEARCH Open Access Metformin therapy in a hyperandrogenic anovulatory mutant murine model with polycystic ovarian syndrome characteristics improves oocyte maturity during superovulation Mary E Sabatini Lankai Guo Maureen P Lynch Joseph O Doyle HoJoon Lee Bo R Rueda and Aaron K Styer Abstract Background Metformin an oral biguanide traditionally used for the treatment of type 2 diabetes is widely used for the management of polycystic ovary syndrome PCOS -related anovulation. Because of the significant prevalence of insulin resistance and glucose intolerance in PCOS patients and their putative role in ovulatory dysfunction the use of metformin was touted as a means to improve ovulatory function and reproductive outcomes in PCOS patients. To date there has been inconsistent evidence to demonstrate a favorable effect of metformin on oocyte quality and competence in women with PCOS. Given the heterogeneous nature of this disorder we hypothesized that metformin may be beneficial in mice with aberrant metabolic characteristics similar to a significant number of PCOS patients. The aim of this study was to gain insight into the in vitro and in vivo effects of metformin on oocyte development and ovulatory function. Methods We utilized metformin treatment in the transgenic ob ob and db db mutant murine models which demonstrate metabolic and reproductive characteristics similar to women with PCOS. Results Metformin did not improve in vitro oocyte maturation nor did it have an appreciable effect on in vitro granulosa cell luteinization progesterone production in any genotype studied. Although both mutant strains have evidence of hyperandrogenemia anovulation and hyperinsulinemia only db db mice treated with metformin had a greater number of mature oocytes and total overall oocytes compared to control. There was no observed impact on body mass or serum .