tailieunhanh - báo cáo hóa học:" Hematopoietic-Prostaglandin D2 synthase through PGD2 production is involved in the adult ovarian physiology"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Hematopoietic-Prostaglandin D2 synthase through PGD2 production is involved in the adult ovarian physiology | Farhat et al. Journal of Ovarian Research 2011 4 3 http content 4 1 3 RESEARCH JOURNAL OF OVARIAN RESEARCH Open Access Hematopoietic-Prostaglandin D2 synthase through PGD2 production is involved in the adult ovarian physiology 1 1 1 Andalib Farhat Pascal Philibert Charles Sultan Francis Poulat Brigitte Boizet-Bonhoure Abstract Background The prostaglandin D2 PGD2 pathway is involved in numerous biological processes and while it has been identified as a partner of the embryonic sex determining male cascade the roles it plays in ovarian function remain largely unknown. PGD2 is secreted by two prostaglandin D synthases Pgds the male-specific lipocalin L -Pgds and the hematopoietic H -Pgds. Methods To study the expression of the Pgds in the adult ovary in situ hybridization were performed. Then to evaluate the role of H-Pgds produced PGD2 in the ovarian physiology adult female mice were treated with HQL-79 a specific inhibitor of H-Pgds enzymatic activity. The effects on expression of the gonadotrophin receptors FshR and LhR steroidogenic genes CypllAl StAR and on circulating progesterone and estradiol were observed. Results We report the localization of H-Pgds mRNA in the granulosa cells from the primary to pre-ovulatory follicles. We provide evidence of the role of H-Pgds-produced PGD2 signaling in the FSH signaling through increased FshR and LhR receptor expression. This leads to the activation of steroidogenic CypllAl and StAR gene expression leading to progesterone secretion independently on other prostanoid-synthetizing mechanisms. We also identify a role whereby H-Pgds-produced PGD2 is involved in the regulation of follicular growth through inhibition of granulosa cell proliferation in the growing follicles. Conclusions Together these results show PGD2 signaling to interfere with FSH action within granulosa cells thus identifying an important and unappreciated role for PGD2 signaling in modulating the balance of proliferation .

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