tailieunhanh - báo cáo hóa học:" Differential expression of aldehyde dehydrogenase 1a1 (ALDH1) in normal ovary and serous ovarian tumors"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Differential expression of aldehyde dehydrogenase 1a1 (ALDH1) in normal ovary and serous ovarian tumors | Penumatsa et al. Journal of Ovarian Research 2010 3 28 http content 3 1 28 RESEARCH JOURNAL OF OVARIAN RESEARCH Open Access Differential expression of aldehyde dehydrogenase 1a1 ALDH1 in normal ovary and serous ovarian tumors 1 1 1 Krishna Penumatsa Seby L Edassery Animesh Barua Michael J Bradaric Judith L Luborsky Abstract Background We showed there are specific ALDH1 autoantibodies in ovarian autoimmune disease and ovarian cancer suggesting a role for ALDH1 in ovarian pathology. However there is little information on the ovarian expression of ALDH1. Therefore we compared ALDH1 expression in normal ovary and benign and malignant ovarian tumors to determine if ALDH1 expression is altered in ovarian cancer. Since there is also recent interest in ALDH1 as a cancer stem cell CSC marker we assessed co-expression of ALDH1 with CSC markers in order to determine if ALDH1 is a potential CSC marker in ovarian cancer. Methods mRNA and protein expression were compared in normal human ovary and serous ovarian tumors using quantitative Reverse-Transcriptase PCR Western blot WB and semi-quantitative immunohistochemistry IHC . ALDH1 enzyme activity was confirmed in primary ovarian cells by flow cytometry FC using ALDEFLUOR assay. Results ALDH1 mRNA expression was significantly reduced p n 5 in malignant tumors compared to normal ovaries and benign tumors. The proportion of ALDH1 cells was significantly lower in malignant tumors n 5 compared to normal ovaries p n 5 and benign tumors p n 5 . ALDH1 cells occurred in the stroma and surface epithelium in normal ovary and benign tumors although surface epithelial expression varied more in benign tumors. Localization of ALDH1 was heterogeneous in malignant tumor cells and little ALDH1 expression occurred in poorly differentiated malignant tumors. In benign tumors the distribution of ALDH1 had features of both normal ovary and malignant tumors. ALDH1 protein .