tailieunhanh - báo cáo hóa học:" Effects of GnRH agonist treatment on steroidogenesis and folliculogenesis in the ovary of cyclic mice"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Effects of GnRH agonist treatment on steroidogenesis and folliculogenesis in the ovary of cyclic mice | Singh and Krishna Journal of Ovarian Research 2010 3 26 http content 3 1 26 RESEARCH JOURNAL OF OVARIAN RESEARCH Open Access Effects of GnRH agonist treatment on steroidogenesis and folliculogenesis in the ovary of cyclic mice Padmasana Singh Amitabh Krishna Abstract Background GnRH analogs both agonist and antagonist have been extensively used for clinical applications following the discovery of its direct effects on ovary. With regard to the direct actions of GnRH agonist on ovary conflicting data are reported. The mechanism through which GnRH agonist affect gonadal functions is still obscure. The aim of present study was thus to investigate the effects of treatment with different doses of GnRH agonist in vivo and in vitro on morphological physiological and functional changes in the ovary of cyclic mice. Methods To find out the effect of GnRH agonist on ovarian activity cyclic mice were treated with different doses for 8 days and its effect on folliculogenesis morphological changes in follicle Estrogen receptor progesterone receptor steroidogenesis circulating progesterone level StAR LH-receptor 3p-HSD luteinization Morphology of corpus luteum and apoptosis caspase-3 PARP were observed. To find the in vitro effects of GnRH agonist with or without LH on ovary of mice changes in the expression of LH-receptor estrogen receptor progesterone receptor 3p-HSD in the ovary and progesterone level in the culture media were investigated. Results GnRH agonist treatment produced significant changes in ovarian mass circulating steroids level and ovarian follicular development steroidogenesis and apoptosis in the mice. GnRH agonist also caused dose dependent histological changes in follicular development and luteinization. The mice treated with different doses of GnRH agonist showed biphasic effects on steroid synthesis due to its effects on ovarian expression of LH-receptor StAR and 3p -hydroxysteroid dehydrogenase proteins. The high dose showed .

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