tailieunhanh - báo cáo hóa học:" Development of a syngeneic mouse model of epithelial ovarian cancer"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Development of a syngeneic mouse model of epithelial ovarian cancer | Quinn et al. Journal of Ovarian Research 2010 3 24 http content 3 1 24 RESEARCH JOURNAL OF OVARIAN RESEARCH Open Access Development of a syngeneic mouse model of epithelial ovarian cancer 1 1 1 2 3 4 Bridget A Quinn Fang Xiao Laura Bickel Lainie Martin Xiang Hua Andres Klein-Szanto Denise C Connolly 1 Abstract Background Most cases of ovarian cancer are epithelial in origin and diagnosed at advanced stage when the cancer is widely disseminated in the peritoneal cavity. The objective of this study was to establish an immunocompetent syngeneic mouse model of disseminated epithelial ovarian cancer EOC to facilitate laboratorybased studies of ovarian tumor biology and preclinical therapeutic strategies. Methods Individual lines of TgMISIIR-TAg transgenic mice were phenotypically characterized and backcrossed to inbred C57BL 6 mice. In addition to a previously described line of EOC-prone mice two lines TgMISIIR-TAg-Low were isolated that express the oncogenic transgene but have little or no susceptibility to tumor development. Independent murine ovarian carcinoma MOVCAR cell lines were established from the ascites of tumor-bearing C57BL 6 TgMISIIR-TAg transgenic mice characterized and tested for engraftment in the following recipient mice 1 severe immunocompromised immunodeficient SCID 2 wild type C57BL 6 3 oophorectomized tumor-prone C57BL 6 TgMISIIR-TAg transgenic and 4 non-tumor prone C57BL 6 TgMISIIR-TAg-Low transgenic. Lastly MOVCAR cells transduced with a luciferase reporter were implanted in TgMISIIR-TAg-Low mice and in vivo tumor growth monitored by non-invasive optical imaging. Results Engraftment of MOVCAR cells by . injection resulted in the development of disseminated peritoneal carcinomatosis in SCID but not wild type C57BL 6 mice. Oophorectomized tumor-prone TgMISIIR-TAg mice developed peritoneal carcinomas with high frequency rendering them unsuitable as allograft recipients. Orthotopic or pseudo-orthotopic implantation of .