tailieunhanh - báo cáo hóa học:" The prosurvival activity of ascites against TRAIL is associated with a shorter disease-free interval in patients with ovarian cancer"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : The prosurvival activity of ascites against TRAIL is associated with a shorter disease-free interval in patients with ovarian cancer | Lane et al. Journal of Ovarian Research 2010 3 1 http content 3 1 1 RESEARCH JOURNAL OF OVARIAN RESEARCH Open Access The prosurvival activity of ascites against TRAIL is associated with a shorter disease-free interval in patients with ovarian cancer Denis Lane Isabelle Matte Claudine Rancourt Alain Piché Abstract Background The production of ascites is a common complication of ovarian cancer. Ascites constitute a unique tumor microenvironment that may affect disease progression. In this context we recently showed that ovarian cancer ascites may protect tumor cells from TRAIL-induced apoptosis. In this study we sought to determine whether the prosurvival effect of ascites affects disease-free intervals. Methods Peritoneal fluids were obtained from 54 women undergoing intra-abdominal surgery for suspected ovarian cancer 44 cancers and 10 benign diseases . The ability of peritoneal fluids to protect from TRAIL was assessed in the ovarian cancer cell line CaOV3 and IC50 were determined. The anti-apoptotic activity of 6 ascites against cisplatin paclitaxel doxorubicin etoposide and vinorelbine was also assessed in CaOV3 cells and the prosurvival activity of two ascites was assessed in 9 primary ovarian cancer cultures. Results Among the 54 peritoneal fluids tested inhibition of TRAIL cytotoxicity was variable. Fluids originating from ovarian cancer were generally more protective than fluids from non-malignant diseases. Most of the 44 ovarian cancer ascites increased TRAIL IC50 and this inhibitory effect did not correlate strongly with the protein concentration in these ascites or the levels of serum CA125 a tumor antigen which is used in the clinic as a marker of tumor burden. The effect of ascites on cisplatin- and paclitaxel-induced cell death was assessed with 4 ascites having inhibitory effect on TRAIL-induced cell death and 2 that do not. The four ascites with prosurvival activity against TRAIL had some inhibitory on cisplatin and or .