tailieunhanh - báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization"

Tham khảo tài liệu 'báo cáo hóa học:" the role of fgf-2 and bmp-2 in regulation of gene induction, cell proliferation and mineralization"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Hughes-Fulford and Li Journal of Orthopaedic Surgery and Research 2011 6 8 http content 6 1 8 JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH RESEARCH ARTICLE Open Access The role of FGF-2 and BMP-2 in regulation of gene induction cell proliferation and mineralization Millie Hughes-Fulford1 2 3 4 Chai-Fei Li4 Abstract Introduction The difficulty in re-growing and mineralizing new bone after severe fracture can result in loss of ambulation or limb. Here we describe the sequential roles of FGF-2 in inducing gene expression cell growth and BMP-2 in gene expression and mineralization of bone. Materials and methods The regulation of gene expression was determined using real-time RTPCR qRTPCR and cell proliferation was measured by thymidine incorporation or fluorescent analysis of DNA content in MC3T3E1 osteoblast-like cells. Photomicroscopy was used to identify newly mineralized tissue and fluorescence was used to quantify mineralization. Results Fibroblast growth factor-2 FGF-2 had the greatest ability to induce proliferation after 24 hours of treatment when compared to transforming growth factor beta TGFp insulin-like growth factor-1 IGF-1 bone morphogenic protein BMP-2 platelet derived growth factor PDGF or prostaglandin E2 PGE2 . We found that FGF-2 caused the most significant induction of expression of early growth response-1 egr-1 fgf-2 cyclo-oxygenase-2 cox-2 tgfb and matrix metalloproteinase-3 mmp-3 associated with proliferation and expression of angiogenic genes like vascular endothelial growth factor A vegfA and its receptor vegfrl. We found that FGF-2 significantly reduced gene expression associated with mineralization . collagen type-1 col1a1 fibronectin fn osteocalcin oc IGF-1 noggin bone morphogenic protein bmp-2 and alkaline phosphatase alp . In contrast BMP-2 significantly stimulated expression of the mineralization associated genes but had little or no effect on gene expression associated with growth. Conclusions The ability of FGF-2