tailieunhanh - Báo cáo y học: "Eotaxin and FGF enhance signaling through an Extracellular signal-related kinase (ERK)-dependent pathway in the pathogenesis of Eosinophilic Esophagitis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Eotaxin and FGF enhance signaling through an Extracellular signal-related kinase (ERK)-dependent pathway in the pathogenesis of Eosinophilic Esophagitis. | Huang et al. Allergy Asthma Clinical Immunology 2010 6 25 http content 6 1 25 ALLERGY ASTHMA CLINICAL IMMUNOLOGY RESEARCH Open Access Eotaxin and FGF enhance signaling through an Extracellular signal-related kinase ERK -dependent pathway in the pathogenesis of Eosinophilic Esophagitis 11 11 111 Jennifer J Huang Jae Won Joh Judy Fuentebella Anup Patel Tammie Nguyen Scott Seki Lisa Hoyte 1 2 2 11 1 Neha Reshamwala Christine Nguyen Anthony Quiros Dorsey Bass Eric Sibley William Berquist Kenneth Cox1 John Kerner1 Kari C Nadeau1 Abstract Background Eosinophilic esophagitis EoE is characterized by the inflammation of the esophagus and the infiltration of eosinophils into the esophagus leading to symptoms such as dysphagia and stricture formation. Systemic immune indicators like eotaxin and fibroblast growth factor were evaluated for possible synergistic pathological effects. Moreover blood cells local tissue and plasma from EoE and control subjects were studied to determine if the localized disease was associated with a systemic effect that correlated with presence of EoE disease. Method Real-time polymerase chain reaction from peripheral blood mononuclear cells PBMC immunohistochemistry from local esophageal biopsies fluid assays on plasma and fluorescence-activated cell sorting on peripheral blood cells from subjects were used to study the systemic immune indicators in newly diagnosed EoE n 35 treated EoE n 9 Gastroesophageal reflux disease GERD n 8 ulcerative colitis n 5 Crohn s disease n 5 and healthy controls n 8 . Result Of the transcripts tested for possible immune indicators we found extracellular signal-regulated kinase ERK Bcl-2 bFGF basic fibroblast growth factor and eotaxin levels were highly upregulated in PBMC and associated with disease presence of EoE. Increased FGF detected by immunohistochemistry in esophageal tissues and in PBMC was correlated with low levels of pro-apoptotic factors Fas Caspase 8 in PBMC from EoE subjects. .