tailieunhanh - Báo cáo khoa học: Hypoxic resistance to articular chondrocyte apoptosis – a possible mechanism of maintaining homeostasis of normal articular cartilage

Hypoxia and hypoxia-related genes are important factors in articular chon-drocytes during cartilage homeostasis and osteoarthritis. We have investi-gated the various apoptotic factors that show significance in synovial fluid obtained from normal and experimental osteoarthritic animal models and have evaluated the effect of hypoxia on articular chondrocyte apoptosis induced by these apoptotic factors. | Hypoxic resistance to articular chondrocyte apoptosis - a possible mechanism of maintaining homeostasis of normal articular cartilage . Seol1 . Lee1 . Lee1 . Lee2 . Kang1 . Kim1 . Kim1 and . Park1 1 Center for Healthcare Technology Development Bio-Safety Research Institute College of Veterinary Medicine Chonbuk National University Jeonju Jeonbuk South Korea 2 Institute of OralBioscience Schoolof Dentistry Chonbuk NationalUniversity Jeonju Jeonbuk South Korea Keywords chondrocytes hypoxia proteasome reactive oxygen species tumour necrosis factor-related apoptosis-inducing ligand TRAIL Correspondence . Park College of Veterinary Medicine Chonbuk NationalUniversity Jeonju Jeonbuk 561-756 South Korea Fax 82 63 270 3780 Tel 82 63 270 3886 E-mail sypark@ Received 21 August 2009 revised 10 October 2009 accepted 20 October 2009 doi Hypoxia and hypoxia-related genes are important factors in articular chondrocytes during cartilage homeostasis and osteoarthritis. We have investigated the various apoptotic factors that show significance in synovial fluid obtained from normal and experimental osteoarthritic animal models and have evaluated the effect of hypoxia on articular chondrocyte apoptosis induced by these apoptotic factors. Mature beagle dogs underwent surgical transections of ligaments and medial meniscectomies to explore the underlying mechanisms of osteoarthritis. Cartilage and synovial fluid obtained from normal animals and those with osteoarthritis were evaluated via pro-teasome inhibition tumour necrosis factor-related apoptosis-inducing ligand TRAIL protein expression mitochondrial transmembrane potential and levels of reactive oxygen species. Canine chondrocytes were exposed to the proteasome inhibitor N-acetyl-Leu-Leu-Norleu-al and treated with recombinant TRAIL protein under normoxic and hypoxic conditions measuring chondrocyte cell viability proteasome activity and levels of .