tailieunhanh - Báo cáo khoa học: Complex gangliosides are apically sorted in polarized MDCK cells and internalized by clathrin-independent endocytosis

Gangliosides are glycosphingolipids mainly present at the outer leaflet of the plasma membrane of eukaryotic cells, where they participate in recogni-tion and signalling activities. The synthesis of gangliosides is carried out in the lumen of the Golgi apparatus by a complex system of glycosyltrans-ferases. | ỊFEBS Journal Complex gangliosides are apically sorted in polarized MDCK cells and internalized by clathrin-independent endocytosis Pilar M. Crespo Natalia von Muhlinen Ramiro Iglesias-Bartolome and Jose L. Daniotti Centro de Investigaciones en Quimica Biologica de Cordoba CIQUIBIC UNC-CONICET Departamento de Quimica Biologica Facultad de Ciencias Quimicas Universidad Nacionalde Cordoba Argentina Keywords gangliosides glycolipids intracellular trafficking MDCK cells polarized cells Correspondence J. L. Daniotti Centro de Investigaciones en Quimica Biologica de Cordoba CIQUIBIC UNC-CONICET Departamento de Quimica Biologica Facultad de Ciencias Quimicas Universidad Nacionalde Cordoba Haya de la Torre y Medina Allende Ciudad Universitaria X5000HUA Cordoba Argentina Fax 54 351 433 4074 Tel 54 351 433 4168 4171 E-mail daniotti@ Received 4 August 2008 revised 21 September 2008 accepted 8 October 2008 doi Gangliosides are glycosphingolipids mainly present at the outer leaflet of the plasma membrane of eukaryotic cells where they participate in recognition and signalling activities. The synthesis of gangliosides is carried out in the lumen of the Golgi apparatus by a complex system of glycosyltransferases. After synthesis gangliosides leave the Golgi apparatus via the lumenal surface of transport vesicles destined to the plasma membrane. In this study we analysed the synthesis and membrane distribution of GD3 and GM1 gangliosides endogenously synthesized by Madin-Darby canine kidney MDCK cell lines genetically modified to express appropriate ganglioside glycosyltransferases. Using biochemical techniques and confocal laser scanning microscopy analysis we demonstrated that GD3 and GM1 after being synthesized at the Golgi apparatus were transported and accumulated mainly at the plasma membrane of nonpolarized MDCK cell lines. More interestingly both complex gangliosides were found to be enriched mainly at the apical domain .

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