tailieunhanh - Báo cáo khoa học: A decade of Cdc14 – a personal perspective Delivered on 9 July 2007 at the 32nd FEBS Congress in Vienna, Austria

In budding yeast, the protein phosphatase Cdc14 is a key regulator of late mitotic events. Research over the last decade has revealed many of its func-tions and today we know that this protein phosphatase orchestrates several aspects of chromosome segregation and is the key trigger of exit from mitosis. | THE IUBMB LECTURE A decade of Cdc14 - a personal perspective Delivered on 9 July 2007 at the 32nd FEBS Congress in Vienna Austria Angelika Amon David H. Koch Institute for Integrative Cancer Research Cambridge MA USA Keywords anaphase Cdc14 cellcycle chromosome segregation FEAR network mitosis mitotic exit mitotic exit network nucleolus phosphatase Correspondence A. Amon David H. Koch Institute for Integrative Cancer Research Howard Hughes Medical Institute Massachusetts Institute of Technology E17-233 40 Ames Street Cambridge MA 02139 USA Fax 1 617 258 6558 Tel 1 617 258 8964 E-mail angelika@ Received 29 July 2008 revised 15 September 2008 accepted 18 September 2008 doi In budding yeast the protein phosphatase Cdc14 is a key regulator of late mitotic events. Research over the last decade has revealed many of its functions and today we know that this protein phosphatase orchestrates several aspects of chromosome segregation and is the key trigger of exit from mitosis. Elucidation of the mechanisms controlling Cdc14 activity through nucleolar sequestration now serves as a paradigm for how regulation of the subcellular localization of proteins regulates protein function. Here I review these findings focusing on how discoveries in my laboratory helped elucidate the function and regulation of Cdc14. Discovery of Cdc14 as the key regulator of exit from mitosis in budding yeast In 1995 my scientific life changed. I was given the unique opportunity to become a Whitehead Fellow. The Whitehead Fellow s Program allows young scientists mostly straight out of graduate school to pursue their independent research program. At the time I was a postdoctoral fellow in Ruth Lehman s laboratory and Ruth had decided to move to New York. I could not go with her and also realized that flies were not my thing and decided to give this a try. But what should I work on As a graduate student in Kim Nasmyth s laboratory I had studied the regulation of .