tailieunhanh - Báo cáo khoa học: Acoustic microfluidic chip technology to facilitate automation of phage display selection

Modern tools in proteomics require access to large arrays of specific bind-ers for use in multiplex array formats, such as microarrays, to decipher complex biological processes. Combinatorial protein libraries offer a solu-tion to the generation of collections of specific binders, but unit operations in the process to isolate binders from such libraries must be automatable to ensure an efficient procedure. | IFEBS Journal Acoustic microfluidic chip technology to facilitate automation of phage display selection Jonas Persson1 Per Augustsson2 Tomas Laurell2 and Mats Ohlin1 1 Department of Immunotechnology Lund University Sweden 2 Department of ElectricalMeasurements Lund University Sweden Keywords acoustic standing wave forces antigenspecific binding microfluidic chip phage display selection Correspondence M. Ohlin Department of Immunotechnology Lund University BMC D13 SE-22184 Lund Sweden Fax 46 4622 24200 Tel 46 4622 24322 E-mail Received 4 July 2008 revised 5 September 2008 accepted 18 September 2008 Modern tools in proteomics require access to large arrays of specific binders for use in multiplex array formats such as microarrays to decipher complex biological processes. Combinatorial protein libraries offer a solution to the generation of collections of specific binders but unit operations in the process to isolate binders from such libraries must be automatable to ensure an efficient procedure. In the present study we show how a microfluidic concept that utilizes particle separation in an acoustic force field can be used to efficiently separate antigen-bound from unbound members of such libraries in a continuous flow format. Such a technology has the hallmarks for incorporation in a fully automated selection system for the isolation of specific binders. doi The potential for recombinant antibodies in various analytical and therapeutic applications has developed substantially over recent years. With new therapeutic targets emerging continuously for various diseases and with the completion of the human genome sequencing project 1 2 extensive efforts are now directed towards understanding how complex sets of gene products are responsible for the many different functions of living cells with respect to both health and disease. The multiplex analysis approach employing large arrays of antibodies is being used to .

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