tailieunhanh - Waste Water Evaluation and Management Part 6

Tham khảo tài liệu 'waste water evaluation and management part 6', kỹ thuật - công nghệ, cơ khí - chế tạo máy phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Formaldehyde Oxidizing Enzymes and Genetically Modified Yeast Hansenula polymorpha Cells in Monitoring and Removal of Formaldehyde 139 The basic bioanalytical characteristics of the bi-enzyme biosensor polarized at 180 mV vs. NHE are presented in Table 9 and Fig. 14. The biosensor-FA reaction obeys typical Michaelis-Menten kinetics. The detection limit was found to be 32 pM while the dynamic range was shown to be linear between and mM FA. The slope of the calibration curve sensitivity and the linear correlation coefficient were 22 Am-2M-1 and respectively. The stability of the FdDH immobilized on the electrode was also evaluated. When the biosensors were stored at 4 0C in phosphate buffer pH the response was linear with a loss of 50 of the activity after 24 h. Dry storage of the immobilized electrode at the same temperature resulted in the complete inactivation of the immobilized enzyme. 200 1 Fig. 14. Calibration curve of the FdDH-DPH-PVP-Os-modified electrode mM NAD mM GSH M phosphate buffer pH E appl 160 mV ml min flow rate The comparison of the developed FA-selective biosensors Tables 9 and 10 represent a brief summary of the published results on the developed microbial and enzyme-based FA biosensors with differend types of signal detection. The amperometric biosensors enzyme- and cell-based work at a very low applied potential compared with other known biosensors zero or 160 vs. 340 610 or 560 mV thus the possible interferences . methanol ethanol acetic acid should be considerably reduced. Different approaches were used for biosensor monitoring FA-dependent cell response 1 analysis of their oxygen consumption rate by using a Clark electrode 2 assay of oxidation of redox mediator at a screen-printed platinum electrode covered by cells entrapped in Ca-alginate gel Khlupova et al. 2007 . 140 Waste Water - Evaluation and Management The dynamic ranges of all described biosensors were of micromolar values. As can be .

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