tailieunhanh - Báo cáo khoa học: "Embryogenesis Jin Quercus petraea"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: Embryogenesis Jin Quercus petraea. | 344s Ann Sci For 1993 50 Suppl 1 344S-350S Elsevier INRA Original article Embryogenesis in Quercus petraea J Jorgensen Lower Saxony Forest Research Institute Department of Forest Tree Breeding 3513 Staufenberg-Escherode Germany Summary Embryogenesis in Quercus petraea was achieved by culture of zygotic embryos and anthers on modified woody plant medium with benzylaminopurine and 2 4 dichlorophenoxyacetic acid. After counting chromosomes and isoenzyme analysis the embryos from anther culture were subdivided into haploid and somatic clones. Protoplasts that had been isolated from haploid and diploid tissues were regenerated to embryos. somatic embryogenesis androgenesis protoplast culture Quercus petraea Resume Embryogenèse Chez Quercus petraea. L embryogenese de Quercus petraea a été réalisée par culture d embryons zygotiques et d antheres sur du milieu WP contenant de la BAP et du 2 4 D. L analyse des isoenzymes et le dénombrement des chromosomes ont montré que les em-bryons issus de culture d antheres étaient subdivisés en clones haplo ides et somatiques. Les proto-plastes Isolds à partir de tissus haploides et diploides ont été régénérés en embryons. embryogenèse somatique androgenèse culture de protoplastes Quercus petraea INTRODUCTION In contrast to the breeding of agricultural and horticultural plants the breeding of oak faces special difficulties 1 inbred lines which help to combine attributes exactly cannot be produced due to the long generation period of oak 2 the rate of rooting in cuttings of older mother trees is quite low Spethmann 1986 so that a sufficient supply of clones of high genetic quality can hardly be achieved 3 the forests cannot be constantly supplied with oak seeds or plants because oak seeds cannot be stored for more than 3-4 years. This situation is quite unsatisfactory when confronting simultaneous increases of forest decline and wood demand. Although at the beginning of this program only shoot cultures had been described for this .