tailieunhanh - Báo cáo khoa học: "Long-term effects of culture establishment from shoot-tip explants in micropropagating oak (Quercus robur L)"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: "Long-term effects of culture establishment from shoot-tip explants in micropropagating oak (Quercus robur L). | Ann Sci For 1994 51 581-588 ẹ Elsevier INRA 581 Original article Long-term effects of culture establishment from shoot-tip explants in micropropagating oak Quercus robur L B Juncker JM Favre Université de Nancy I Faculte des Sciences Laboratoire de Biologie des Ligneux BP 239 54506 Vandceuvre cedex France Received 11 December 1992 accepted 2 February 1994 Summary This paper describes a method of in vitro culture establishment from shoot-tip explants taken from juvenile and mature plant material for oak table I . The cultures established from shoot-tips were then compared with cultures derived from nodal explants for decontamination their initial reactivity and their potential for long-term propagation. For the decontamination the results showed that the use of shoot-tip explants is useful only when culture establishement must be made directly from source-plants growing in situ table II . Otherwise the use of nodal explants taken from source-plants that are maintained under active growth and controlled sanitary conditions is more advisable due to a better initial reactivity. As regards the potential for long-term propagation the culture establishment from shoot-tips appeared truly interesting only in the case of recalcitrant clones and or insufficient optimization of the culture methods fig 1 . However this positive effect attenuated after a 6-7 month culture period and the clonal effects and the management of the media became the determining factors of the culture behaviour whatever the initial explant used fig 2 . shoop-tip explant decontamination I long-term propagation Quercus robur L mature plant material juvenile plant material Resume Effets à long terme de I introduction in vitro à partir de méristèmes sur la micropropagation du chêne Quercus roburL . L article décrit Chez le chêne les conditions d obtention d un clonage in vitro à partir de méristèmes prélevés sur du materiel juvenile et sur du materiel mature tableau I . II compare ensuite sur le plan de la