tailieunhanh - Báo cáo khoa học: "Micropropagation of the pine hybrid Pinus brutia (Ten) x Pinus halepensis (Mill) by culturing fascicle shoots"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: Micropropagation of the pine hybrid Pinus brutia (Ten) x Pinus halepensis (Mill) by culturing fascicle shoots. | 175 Ann Sci For 1994 51 175-182 Elsevier INRA Original article Micropropagation of the pine hybrid Pinus brutia Ten X Pinus halepensis Mill by culturing fascicle shoots A Scaltsoyiannes 1 K Panetsos 1 A Economou 2 p Tsoulpha 1 Laboratory of Forest Genetics Department of Forestry and Natural Environment Aristotelian University of Thessaloniki 2 Laboratory of Floriculture Department of Agriculture Aristotelian University of Thessaloniki 54006 Greece Received 16 February 1993 accepted 26 October 1993 Summary Fascicle shoots proved to be an ideal plant material for micropropagation of the pine hybrid p brutia Ten X p halepensis Mill . This could be possibly attributed to their morphological and physiological state. Induced fascicle shoots of 4-yr-old seedlings were used as explants. Their induction was achieved by spraying once with the new herbicide Arsenal 1 000 mg l 1 . First explants were elongated in vitro on LP medium and then transferred to the multiplication stage. Multiplication was accomplished by decapitating quick-dipping in mM BA and inoculating the explants on BIMI medium. On the induced first-generation shoots the same procedure was applied in order to obtain second-generation shoots and then these were proceeded to the rooting and acclimatization stages. In particular when microcuttings were pretreated with pM IBA pM NAA agar w v sucrose w v for 7 d and then transferred to greenhouse conditions a good root system was developed within an 8-12-week period. A large variation in rootability was noted between clones. The above method may be proved to be efficient for clones that exhibit high rooting ability. p brutia Ten X p halepensis Mill I induction of fascicle shoots micropropagation clonal variation Resume Micropropagation de I hybride du pin p brutia Ten X p halepensis Mill au moyen de culture des pousses fasciculaires. Les pousses fasciculaires constituent un materiel vegetal ideal pour la micropropagation de I hybride .