tailieunhanh - Properties and Applications of Silicon Carbide Part 13

Tham khảo tài liệu 'properties and applications of silicon carbide part 13', kỹ thuật - công nghệ, cơ khí - chế tạo máy phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | 352 Properties and Applications of Silicon Carbide reporter system was developed which uses the green fluorescent protein GFP from jellyfish Aquorea victoria . This reporter gene does not require a destructive staining procedure and allows direct viewing of gene expression in living plant tissue. Similar to the GUS reporter system gfp can be introduced into plants using the Ti-plasmid. Following T-DNA transfer GFP can be viewed directly in living tissues with blue light excitation. The GFP reporter system permits detection of labeled protein within cells and monitoring plant cells expressing gfp directly within growing plant tissue Haseloff Siemering 1998 . Since the gfp gene was first reported as a useful marker for gene expression in Escherichia coli and Caenorhabditis elegans it has been modified by several laboratories to suit different purposes to include elimination of a cryptic intron alteration in codon usage changes in the chromophore leading to different excitation and emission spectra targeting to the endoplasmic reticulum ER and mitochondria and understanding the morphology and dynamics of the plant secretory pathway Brandizzi et al. 2004 . GFP has been used as a reporter system for identifying transformation events in Arabidopsis thaliana apple rice sugarcane maize lettuce tobacco soybean oat onion wheat leek and garlic Eady et al. 2005 . The GFP reporter system has also been used for identifying successful plastid transformation events in potato. The gfp gene has successfully been used as a scorable marker to evaluate plant transformation efficiency using Agrobacterium tumefaciens particle bombardment and whisker mediated gene transfer. The gene could be expressed as early as h following introduction and since its detection is nondestructive gfp expression could be followed over extended periods of time. GFP has also been used as a reporter to analyze the compartmentalization and movement of proteins over time in living plant cells using confocal

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