tailieunhanh - Báo cáo khoa học: "Reinvigoration treatments for the micropropagation of mature chestnut trees"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: "Reinvigoration treatments for the micropropagation of mature chestnut trees. | Ann Set For 1997 54 359-370 Elsevier Inra 359 Original article Reinvigoration treatments for the micropropagation of mature chestnut trees MC Sanchez A Ballester AM Vieitez Institute de Investigaciones Agrobiological de Galicia CSIC Apartado 122 5080 Santiago de Compostela Spain Received 20 November 1995 accepted 1 1 June 1996 Summary - Crown material from five adult chestnut trees was given different reinvigoration treatments such as 6-benzylaminopurine BA applications spray or pulse to forced cuttings and juvenile grafting alone or combined with BA sprays then used for the establishment in vitro. The in vitro performance in terms of establishment multiplication and rooting of both untreated and treated material was compared. Grafting alone or in combination with BA spray greatly increased the in vitro reactivity of crown-derived explants. By combining in vivo pretreatments and a horizontal reculturing system crown-derived microshoots exhibited maximum rooting rates similar to those found for cultures from basal shoots of the same tree in previous work. chestnut horizontal reculture micropropagation mature trees partial rejuvenation Resume - Traitements de rajeunissement de châtaigniers adultes. Pour faciliter 1 établissement in vitro du Chataignier differents traitements tels que des applications de 6-benzylaminopurine BA pulverisation OU trempage de 2 h ie greffage sur porte-greffe juvenile seul OU combine avec des pulverisations de BA ont été appliques au materiel prélevé dans la couronne de cinq châtaigniers adultes. On a compare le comportement durant rétablissement la multiplication el 1 cnracinement in vitro du materiel non traité témoin et du materiel rajeuni. Durant Tétablissement in vitro la réac-tivité du materiel témoin a été relativement faible pour tous les clones variations entre 0 et 22 . Le greffage seul OU combine avec des pulverisations de BA augmente significativement la réactivité des explants provenant de la cime des arbres atteignant 94 pour