tailieunhanh - báo cáo hóa học:" G0/G1 arrest and apoptosis induced by SARS-CoV 3b protein in transfected cells"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : G0/G1 arrest and apoptosis induced by SARS-CoV 3b protein in transfected cells | Virology Journal BioMed Central Short report Open Access G0 GI arrest and apoptosis induced by SARS-CoV 3 b protein in transfected cells Xiaoling Yuan Yajun Shan Zhenhu Zhao Jiapei Chen and Yuwen Cong Address Department of Pathophysiology Beijing Institute of Radiation Medicine Beijing 100850 China Email XiaolingYuan - xiaolingyuan@ Yajun Shan - shanyajun0207@ Zhenhu Zhao - zhaozhenhu@ Jiapei Chen - chenjp@ Yuwen Cong - congyw@ Corresponding author Published 17 August 2005 Received 30 April 2005 Accepted 17 August 2005 Virology Journal 2005 2 66 doi 1743-422X-2-66 This article is available from http content 2 1 66 2005 Yuan et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Severe Acute Respiratory Syndrome coronavirus SARS-CoV cause of the life-threatening atypical pneumonia infects many organs such as lung liver and immune organ and induces parenchyma cells apoptosis and necrosis. The genome of SARS-CoV not closely related to any of the previously characterized coronavirus encodes replicase and four major structural proteins and a number of non-structural proteins. Published studies suggest that some non-structural proteins may play important roles in the replication virulence and pathogenesis of viruses. Among the potential SARS-CoV non-structural proteins 3b protein ORF4 is predicted encoding 154 amino acids lacking significant similarities to any known proteins. Till now there is no report about the function of 3b protein. In this study 3b gene was linked with the EGFP tag at the C- terminus. Through cell cycle analysis it was found that over-expression of 3b-EGFP protein in Vero 293 and COS-7 cells could induce cell cycle arrest