tailieunhanh - báo cáo hóa học:" Possible active origin of replication in the double stranded extended form of the left terminus of LuIII and its implication on the replication model of the parvovirus"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Possible active origin of replication in the double stranded extended form of the left terminus of LuIII and its implication on the replication model of the parvovirus | Virology Journal BioMed Central Research Open Access Possible active origin of replication in the double stranded extended form of the left terminus of LuIII and its implication on the replication model of the parvovirus Nanette Diffoot-Carlo Lisandra Vélez-Pérez and Idaris de Jesús-Maldonado Address Department of Biology University of Puerto Rico . Box 9012 Mayaguez Puerto Rico 00680 Email Nanette Diffoot-Carlo - ndiffoot@ Lisandra Vélez-Pérez - lisandravp@ Idaris de Jesús-Maldonado - idarisdejesus@ Corresponding author Published 31 May 2005 Received 14 April 2005 Accepted 3 1 May 2005 Virology Journal 2005 2 47 doi l 743-422X-2-47 This article is available from http content 2 1 47 2005 Diffoot-Carlo et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The palindromic termini of parvoviruses have proven to play an essential role as origins of replication at different stages during the replication of their viral genome. Sequences from the left-end telomere of MVM form a functional origin on one side of the dimer replicative form intermediate. In contrast the right-end origin can operate in its closed replicative form hairpin configuration or as a fully duplex linear sequence derived from either arm of a palindromic tetramer intermediate. To study the possibility that the LuIII left hairpin has a function in replication comparable to that described for MVM the replication of a minigenome containing two copies of the LuIII left terminus LuIII Lt-Lt was studied. Results The data presented demonstrates that LuIII Lt-Lt was capable of replicating when NS1 helper functions were provided in trans. This extended hairpin capable of acting as an origin of .