tailieunhanh - báo cáo hóa học:" Amphotropic murine leukaemia virus envelope protein is associated with cholesterol-rich microdomains"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Amphotropic murine leukaemia virus envelope protein is associated with cholesterol-rich microdomains | Virology Journal BioMed Central Research Amphotropic murine leukaemia virus envelope protein is associated with cholesterol-rich microdomains Christiane Beer1 2 Lene Pedersen2 and Manfred Wirth 1 Open Access Address 1Molecular Biotechnology German Research Centre for Biotechnology GBF Mascheroder Weg 1 D-38124 Braunschweig Germany and 2Institute of Clinical Medicine and Department of Molecular Biology University of Aarhus Aarhus Denmark Email Christiane Beer - chb@ Lene Pedersen - lp@ Manfred Wirth - mwi@ Corresponding author Published 19 April 2005 Received 31 March 2005 Accepted 19 April 2005 Virology Journal 2005 2 36 doi 186 1743-422X-2-36 This article is available from http content 2 1 36 2005 Beer et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Cholesterol-rich microdomains like lipid rafts were recently identified as regions within the plasma membrane which play an important role in the assembly and budding of different viruses . measles virus and human immunodeficiency virus. For these viruses association of newly synthesized viral proteins with lipid rafts has been shown. Results Here we provide evidence for the association of the envelope protein Env of the 4070A isolate of amphotropic murine leukaemia virus A-MLV with lipid rafts. Using density gradient centrifugation and immunocytochemical analyses we show that Env co-localizes with cholesterol ganglioside GM1 and caveolin-1 in these specific regions of the plasma membrane. Conclusions These results show that a large amount of A-MLV Env is associated with lipid rafts and suggest that cholesterol-rich microdomains are used as portals for the exit of A-MLV. Background Cholesterol-rich .