tailieunhanh - Báo cáo y học: "Growth of Microorganisms in Total Parenteral Nutrition Solutions Containing Lipi"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Growth of Microorganisms in Total Parenteral Nutrition Solutions Containing Lipid. | Int. J. Med. Sci. 2010 7 101 International Journal of Medical Sciences 2010 7 3 101-109 Ivyspring International Publisher. All rights reserved Research Paper Growth of Microorganisms in Total Parenteral Nutrition Solutions Containing Lipid Takashi Kuwahara10 Kazuyuki Shimono1 Shinya Kaneda1 Takumi Tamura1 Masao Ichihara2 Yoshifumi Nakashima1 1. Preclinical Assessment Department Otsuka Pharmaceutical Factory Inc. Tokushima Japan. 2. Research and Development Center Otsuka Pharmaceutical Factory Inc. Tokushima Japan. ISI Corresponding author Takashi Kuwahara . Preclinical Assessment Department Otsuka Pharmaceutical Factory Inc. 115 Tateiwa Naruto Tokushima 772-8601 Japan. Telephone 81 88 685 1151 Ext. 678 Fax 81 88 684 0553 E-mail kuwahat2@ Received Accepted Published Abstract Background To identify the microorganisms that can grow rapidly in total parenteral nutrition TPN solutions we investigated the growth of the major causes of catheter-related blood stream infection Staphylococcus aureus Serratia marcescens Bacillus cereus and Candida albicans in TPN solutions containing lipid. Methods The pH value of a TPN solution containing lipid pH containing 20 ppm of NaHSO3 was adjusted by the addition of HCl to or . The pH value of another TPN solution containing 400 ppm of NaHSO3 was adjusted by the addition of NaOH to or . A specific number of each microorganism was added to 10 mL of each test solution and incubated at room temperature. The number of microorganisms was counted as colony forming units at 0 24 and 48 hrs later. Results C albicans increased similarly at any pH values in the TPN solution. The bacterial species also increased rapidly at in the solution containing 20 ppm of NaHSO3 but growth was suppressed as the pH value was reduced with growth halted at . However these bacterial species did not increase even at in the other solution containing 400 ppm of .