tailieunhanh - Báo cáo y học: "Growth of Microorganisms in Total Parenteral Nutrition Solutions Without Lipid"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Growth of Microorganisms in Total Parenteral Nutrition Solutions Without Lipid. | Int. J. Med. Sci. 2010 7 43 International Journal of Medical Sciences 2010 7 1 43-47 Ivyspring International Publisher. All rights reserved Research Paper Growth of Microorganisms in Total Parenteral Nutrition Solutions Without Lipid Takashi Kuwahara10 Shinya Kaneda1 Kazuyuki Shimono1 Yoshifumi Inoue2 1. Preclinical Assessment Department Otsuka Pharmaceutical Factory Inc. 115 Tateiwa Naruto Tokushima 772-8601 Japan. 2. Surgery Kawasaki Hospital 3-3-1 Higashiyamamachi Hyougo-ku Kobe 652-0042 Japan ISI Corresponding author Takashi Kuwahara . Preclinical Assessment Department Otsuka Pharmaceutical Factory Inc. 115 Tateiwa Naruto Tokushima 772-8601 Japan. Telephone 81 88 685 1151 Ext. 678 Fax 81 88 684 0553 E-mail kuwahat2@ Received Accepted Published Abstract Background To identify the microorganisms that can grow rapidly in total parenteral nutrition TPN solutions we investigated the growth of the major causes of catheter-related blood stream infection Staphylococcus aureus Serratia marcescens Bacillus cereus and Candida albicans in TPN solutions without lipid. Methods Experiment 1 A commercial TPN solution without lipid containing multivitamins was used. A specific number of each test microorganism was added to each 10 mL of the TPN solution and incubated at room temperature. An aliquot of test solution was sampled and inoculated to SCD agar plates at 0 24 and 48 hrs after the addition of the microorganisms. The number of microorganisms was counted as colony forming units. Experiment 2 The other 2 commercial TPN solutions without lipid were supplemented with multivitamins. The pH values of the solutions were adjusted to about or using mol L NaOH. The addition of microorganisms incubation and counting were performed in the same manner. Results Experiment 1 S. aureus S. marcescens and B. cereus did not increase in the TPN solution without lipid containing multivitamins but C. albicans