tailieunhanh - Báo cáo y học: "Autofluorescent Proteins as Photosensitizer in Eukaryonte"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Autofluorescent Proteins as Photosensitizer in Eukaryontes. | Int. J. Med. Sci. 2009 6 365 International Journal of Medical Sciences 2009 6 6 365-373 Ivyspring International Publisher. All rights reserved Research Paper Autofluorescent Proteins as Photosensitizer in Eukaryontes Waldemar Waldeck1 Gabriele Mueller1 Manfred Wiessler2 Manuela Brom3 Katalin Tóth1 and Klaus Braun2 s 1. German Cancer Research Center Dept. of Biophysics of Macromolecules INF 580 D-69120 Heidelberg Germany 2. German Cancer Research Center Dept. of Medical Physics in Radiology INF 280 D-69120 Heidelberg Germany 3. German Cancer Research Center Core Facility Light Microscopy INF 581 D-69120 Heidelberg Germany H Correspondence to Dr. Klaus Braun German Cancer Research Center DKFZ Dept. of Medical Physics in Radiology Im Neuenherner Feld 280 D-69120 Heidelberg Germany. Tel 49 6221 42 2495 Fax 491 6221 42 3326. Received Accepted Published Abstract Since the discovery of the green fluorescent green protein GFP in 1961 many variants of fluorescent proteins FP were detected. The importance was underlined by the Nobel price award in chemistry 2008 for the invention application and development of the GFP by Shimomura Chalfie and Tsien. GFP first described by Shimomura now is indispensible in the scientific daily life. Since then and also in future fluorescent proteins will lead to new applications as reporters in cell biology. Such FPs can absorb visible day-light and predominantly one variant of the red fluorescent protein the KillerRed protein KRED emits active electrons producing reactive oxygen species ROS leading to photokilling processes in eukaryotes. KRED can be activated by daylight as a photosensitizing agent. It is quite obvious that the KRED s expression and localization is critical with respect to damage mutation and finally killing of eukaryotic cells. We found evidence that the KRED s cytotoxicity is ascendantly location-dependent from the cell membrane over the nuclear lamina to the chromatin in .