tailieunhanh - Báo cáo y học: "Parvovirus B19 Nonstructural Protein-Induced Damage of Cellular DNA and Resultant Apoptosis"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Parvovirus B19 Nonstructural Protein-Induced Damage of Cellular DNA and Resultant Apoptosis. | Int. J. Med. Sci. 2011 8 88 Research Paper International Journal of Medical Sciences 2011 8 2 88-96 Ivyspring International Publisher. All rights reserved. Parvovirus B19 Nonstructural Protein-Induced Damage of Cellular DNA and Resultant Apoptosis Brian D. Poole1 2 Violetta Kivovich1 3 4 5 Leona Gilbert4 5 and Stanley J. Naides1 5 6 H 1. Huck Institute for Life Sciences Pennsylvania State University College of Medicine Milton S. Hershey Medical Center Hershey PA USA 2. Current Department of Microbiology and Molecular Biology Brigham Young University Provo UT USA 3. MD PhD Program Pennsylvania State University College of Medicine Milton S. Hershey Medical Center Hershey PA USA 4. Chemical Biology Division Department of Biological and Environmental Science University of Jyvaskyla Jyvaskyla Finland 5. Division of Rheumatology Department of Medicine Pennsylvania State University College of Medicine Milton S. Hershey Medical Center Hershey PA USA 6. Current Quest Diagnostics Nichols Institute San Juan Capistrano CA USA H Corresponding author Stanley J. Naides MD Immunology Quest Diagnostics Nichols Institute 33608 Ortega Highway San Juan Capistrano CA 92690. Tel. 949 728-4578 fax 949 728-7852 email Received Accepted Published Abstract Parvovirus B19 is a widespread virus with diverse clinical presentations. The viral non-structural protein NS1 binds to and cleaves the viral genome and induces apoptosis when transfected into nonpermissive cells such as hepatocytes. We hypothesized that the cytotoxicity of NS1 in such cells results from chromosomal DNA damage caused by the DNA-nicking and DNA-attaching activities of NS1. Upon testing this hypothesis we found that NS1 covalently binds to cellular DNA and is modified by PARP an enzyme involved in repairing single-stranded DNA nicks. We furthermore discovered that the DNA nick repair pathway initiated by poly ADPribose polymerase and the DNA repair .

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