tailieunhanh - Báo cáo y học: "The characterisation of mucin in a mature ovarian teratoma occurring in an eight year old patient"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: The characterisation of mucin in a mature ovarian teratoma occurring in an eight year old patient. | Int. J. Med. Sci. 2007 4 115 International Journal of Medical Sciences ISSN 1449-1907 2007 4 2 115-123 Ivyspring International Publisher. All rights reserved Research Paper The characterisation of mucin in a mature ovarian teratoma occurring in an eight year old patient Anwar Suleman Mall 1 Marilyn Tyler 1 Zoe Lotz 1 Alan Davidson 1 Jerry Rodrigues 4 George van der Watt3 Delawir Kahn 1 Dhirendra Govender 2 1. Departments of Surgery Groote Schuur and Red Cross Hospitals University of Cape Town Cape Town South Africa 2. Anatomical Pathology Groote Schuur Hospital University of Cape Town Cape Town South Africa 3. Chemical Pathology Groote Schuur Hospital University of Cape Town Cape Town South Africa 4. Department of Molecular and Cell Biology University of Cape Town Cape Town South Africa Correspondence to Anwar Suleman Mall PhD Department of Surgery University of Cape Town J-Floor Old Main Building Groote Schuur Hospital OBSERVATORY 7925 SOUTH AFRICA. Tel 27 21 406-6224 Fax 27 21 448-6461 Email . Received Accepted Published Introduction The presence of MUC5AC M1 antigen and MUC6 have previously been found in ovarian mucinous cyst. We characterized the mucins in the crude mucus and tissue of a mature ovarian teratoma in an 8 year old girl. Materials and Methods Mucins were purified from crude mucus by density gradient ultra-centrifugation in CsCl and analysed by gel-filtration and SDS-PAGE analysis. Mucin identification and expression was by western blotting and immunohistochemistry. Results Histology showed a tumour with solid and cystic areas with the cysts lined by colonic and respiratory mucosae. Equal volumes of sol and gel phases of approximately of crude mucus were obtained. Gel filtration and SDS-PAGE analyses suggested that the mucin was mainly of the large polymeric type which dissociated upon reduction of disulphide bonds with DTT. The colonic and respiratory epithelia .

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