tailieunhanh - báo cáo hóa học:" Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài :Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro | Virology Journal BioMed Central Research Open Access Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro Anthony Simmons - 1-3 Robert P Whitehead2 Andrey A Kolokoltsov3 and Robert A Davey-3 Address Department of Pediatrics University of Texas Medical Branch Galveston Texas USA 2Department of Internal Medicine University of Texas Medical Branch Galveston Texas USA and 3Department of Microbiology Immunology University of Texas Medical Branch Galveston Texas USA Email Anthony Simmons - ansimmon@ Robert P Whitehead - rpwhiteh@ Andrey A Kolokoltsov - aakoloko@ Robert A Davey - radavey@ Corresponding author fEqual contributors Published 28 February 2006 Received 10 January 2006 Accepted 28 February 2006 VirologyJournal2006 3 8 doi I743-422X-3-8 This article is available from http content 3 1 8 2006Simmons et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Genetic redirection of lymphocytes that have been genetically engineered to recognize antigens other than those originally programmed in their germlines is a potentially powerful tool for immunotherapy of cancers and potentially also of persistent viral infections. The basis for this procedure is that both cancers and some viruses have developed strikingly similar mechanisms of evading attacks by host immune mechanisms. To redirect human peripheral blood lymphocytes PBLs with a chimeric T cell receptor chTCR so that they recognize a new target requires a high degree of transfection efficiency a process that is regarded as technically .