tailieunhanh - Báo cáo y học: "Skeletal muscle sodium glucose co-transporters in older adults with type 2 diabetes undergoing resistance training"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Skeletal muscle sodium glucose co-transporters in older adults with type 2 diabetes undergoing resistance training. | Int. J. Med. Sci. 2006 3 84 Research paper International Journal of Medical Sciences ISSN 1449-1907 2006 3 3 84-91 2006 Ivyspring International Publisher. All rights reserved Skeletal muscle sodium glucose co-transporters in older adults with type 2 diabetes undergoing resistance training Francisco Castaneda1 Jennifer E. Layne2 and Carmen Castaneda2 1. Max Planck Institute for Molecular Physiology Dortmund Germany. 2. Nutrition Exercise Physiology and Sarcopema Laboratory Jean Mayer . Department of Agriculture USDA Human Nutrition Research Center on Aging Tufts University Boston MA uSa. Corresponding address Francisco Castaneda . Max Planck Institute for Molecular Physiology Otto-Hahn-Str. 11 44227 Dortmund Germany. Telephone 49 231 133-2222. Fax 49 231 133-2699. E-mail Received Accepted Published We examined the expression of the sodium-dependent glucose co-transporter system hSGLT3 in skeletal muscle of Hispanic older adults with type 2 diabetes. Subjects 65 8 yr were randomized_to resistance training 3x wk n 13 or standard of care controls n 5 for 16 weeks. Skeletal muscle hSGLT3 and GLUT4 mRNA transcript levels were determined by real time RT-PCR. hSGLT3 transcripts increased by a factor of ten following resistance training compared to control subjects P . There were no differences in GLUT4 mRNA expression levels between groups. Protein expression levels of these transporters were confirmed by immunohistochemistry and Western blotting. hSGLT3 after resistance exercise was found not to be co-localized with the nicotinic acetylcholine receptor. The change in hSGLT3 transcript levels in the vastus lateralis muscle was positively correlated with glucose uptake as measured by the change in muscle glycogen stores r P and with exercise intensity as measured by the change in muscle strength r P . Group assignment was be the only independent .