tailieunhanh - báo cáo hóa học:" Two dimensional VOPBA reveals laminin receptor (LAMR1) interaction with dengue virus serotypes 1, 2 and 3"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Two dimensional VOPBA reveals laminin receptor (LAMR1) interaction with dengue virus serotypes 1, 2 and 3 | Virology Journal BioMed Central Research Open Access Two dimensional VOPBA reveals laminin receptor LAMR1 interaction with dengue virus serotypes 1 2 and 3 Phaik Hooi Tio Wan Wui Jong and Mary Jane Cardosa Address Institute of Health and Community Medicine Universiti Malaysia Sarawak 94300 Kota Samarahan Sarawak Malaysia Email Phaik Hooi Tio - phtio@ WanWui Jong - ww_jong@ Mary Jane Cardosa - janecardosa@ Corresponding author Published 25 March 2005 Received 24 February 2005 Accepted 25 March 2005 Virology Journal 2005 2 25 doi 1743-422X-2-25 This article is available from http content 2 1 25 2005 Tio et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The search for the dengue virus receptor has generated many candidates often identified only by molecular mass. The wide host range of the viruses in vitro combined with multiple approaches to identifying the receptor s has led to the notion that many receptors or attachment proteins may be involved and that the different dengue virus serotypes may utilize different receptors on the same cells as well as on different cell types. Results In this study we used sequential extraction of PS Clone D cell monolayers with the detergent P-octylglucopyranoside followed by sodium deoxycholate to prepare a cell membranerich fraction. We then used 2 dimensional 2D gel electrophoresis to separate the membrane proteins and applied a modified virus overlay protein binding assay VOPBA to show that dengue virus serotypes I 2 and 3 all interact with the 37 kDa 67 kDa laminin receptor a common non-integrin surface protein on many cell types. Conclusion At least 3 of the 4 dengue serotypes interact with the 37 kDa