tailieunhanh - Báo cáo y học: "Effect of antibodies on the expression of Plasmodium falciparum circumsporozoite protein gene"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Effect of antibodies on the expression of Plasmodium falciparum circumsporozoite protein gene. | Int. J. Med. Sci. 2006 3 7 Research paper International Journal of Medical Sciences ISSN 1449-1907 2006 3 1 7-10 2006 Ivyspring International Publisher. All rights reserved Effect of antibodies on the expression of Plasmodium falciparum circumsporozoite protein gene B S Jesuíno C Casimiro V E do Rosário and H Silveira Centro de Malária e Outras Doenẹas Tropicais UEI Malária Institute de Higiene e Medicina Tropical Universidade Nova de Lisboa Rua da Junqueira 96 1349-008 Lisbon Portugal Corresponding address Henrique Silveira e-mail hsilveira@. Tel 351 21 3652657 Fax 351 21 3622458 Received Accepted Published Antibodies are known to play an important role in the control of malaria infection. However they can modulate parasite development enhancing infection. The effect of anti-Plasmodium antibodies on the expression of circumsporozoite protein gene csp was investigated. Plasmodium falciparum 3D7 in vitro cultures were submitted to i anti- circumsporoZoite protein monoclonal antibody anti-CSP-mAb 1pg ml ml ml and pg ml and ii purified IgG Fab fragment from a pool of malaria patients 1mg ml and 1pg ml and compared to control cultures. After 24h the number of ring infected erythrocytes was determined in order to calculate invasion efficacy. At 48h culture supernatant was collected and the amount of circumsporozoite protein determined by ELISA parasitaemia was calculated and cells were processed for RNA preparation. Expression of csp gene was quantified using Real time RT-PCR. There was an increase in parasite growth when treated with lower anti-CSP-mAb concentration which was associated with lower csp expression while 1pg ml anti-CSP-mAb treatment presented a growth inhibitory effect accompanied by high csp expression. Key words Plasmodium falciparum circumsporozoite protein erythrocyte invasion 1. INTRODUCTION Recognition of pathogen molecules by antibodies leads to initiation of immune .