tailieunhanh - Báo cáo y học: "Polysaccharides from the root of Angelica sinensis protect bone marrow and gastrointestinal tissues against the cytotoxicity of cyclophosphamide in mice"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Polysaccharides from the root of Angelica sinensis protect bone marrow and gastrointestinal tissues against the cytotoxicity of cyclophosphamide in mice. | Int. J. Med. Sci. 2006 3 1 Research paper International Journal of Medical Sciences ISSN 1449-1907 2006 3 1 1-6 2006 Ivyspring International Publisher. All rights reserved Polysaccharides from the root of Angelica sinensis protect bone marrow and gastrointestinal tissues against the cytotoxicity of cyclophosphamide in mice Marco K. C. Hui William K. K. Wu Vivian Y. Shin Wallace H. L. So and Chi Hin Cho Centre of Infection and Immunology and Department of Pharmacology Faculty of Medicine The University of Hong Kong Hong Kong China Corresponding address Prof. . Cho Department of Pharmacology The University of Hong Kong 21 Sassoon Road Hong Kong China. Email chcho@ Telephone 852-2819-9250 Fax 852-2817-0859 Received Accepted Published Cyclophosphamide CY is a cytostatic agent that produces systemic toxicity especially on cells with high proliferative capacity While polysaccharides from Angelica sinensis AP have been shown to increase the turnover of gastrointestinal mUcosal and hemopoietic stem cells. It is not known whether AP has an effect on CY-induced cytotoxicity on bone marrow and gastrointestinal tract. In this study we assessed the protective actions of AP on CY-induced leukopenia and proliferative arrest in the gastroduodenal mucosa in mice. Subcutaneous injection of CY 200 mg kg provoked dramatic decrease in white blood cell WBC count and number of blood vessels and proliferating cells in both the gastric and duodenal mucosae. Subcutaneous injection of AP significantly promoted the recovery from leukopenia and increased number of blood vessels and proliferating cells in both the gastric and duodenal tissues. Western blotting revealed that CY significantly down-regulated the protein expression of vascular endothelial growth factor VEGF c-Myc and ornithine decarboxylase ODC in gastric mucosae but had no effect on epidermal growth factor EGF expression. AP also reversed the dampening effect of CY on