tailieunhanh - báo cáo hóa học:" Soilborne wheat mosaic virus (SBWMV) 19K protein belongs to a class of cysteine rich proteins that suppress RNA silencing"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Soilborne wheat mosaic virus (SBWMV) 19K protein belongs to a class of cysteine rich proteins that suppress RNA silencing | Virology Journal BioMed Central Research Open Access Soilborne wheat mosaic virus SBWMV 19K protein belongs to a class of cysteine rich proteins that suppress RNA silencing Jeannie Te1 Ulrich Melcher2 Amanda Howard1 and Jeanmarie Verchot-Lubicz 1 Address department of Entomology and Plant Pathology Oklahoma State University Stillwater OK 74078 USA and department of Biochemistry and Molecular Biology Oklahoma State University Stillwater OK 74078 USA Email Jeannie Te - te@ Ulrich Melcher - biocukm@ Amanda Howard - howardar@ Jeanmarie Verchot-Lubicz - verchot@ Corresponding author Published 01 March 2005 Received 14 December 2004 Accepted 01 March 2005 Virology Journal 2005 2 18 doi 1743-422X-2-18 This article is available from http content 2 1 18 2005 Te et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Amino acid sequence analyses indicate that the Soilborne wheat mosaic virus SBWMV 19K protein is a cysteine-rich protein CRP and shares sequence homology with CRPs derived from furo- hordei- peclu- and tobraviruses. Since the hordei- and pecluvirus CRPs were shown to be pathogenesis factors and or suppressors of RNA silencing experiments were conducted to determine if the SBWMV 19K CRP has similar activities. The SBWMV 19K CRP was introduced into the Potato virus X PVX viral vector and inoculated to tobacco plants. The SBWMV 19K CRP aggravated PVX-induced symptoms and restored green fluorescent protein GFP expression to GFP silenced tissues. These observations indicate that the SBWMV 19K CRP is a pathogenicity determinant and a suppressor of RNA silencing. Background Viruses survive in their hosts either by evading or countering host defenses. .