tailieunhanh - báo cáo hóa học:" The immunological potency and therapeutic potential of a prototype dual vaccine against influenza and Alzheimer’s disease"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : The immunological potency and therapeutic potential of a prototype dual vaccine against influenza and Alzheimer’s disease | Davtyan et al. Journal of Translational Medicine 2011 9 127 http content 9 1 127 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access The immunological potency and therapeutic potential of a prototype dual vaccine against influenza and Alzheimer s disease 1 3 3 2 2 Hayk Davtyan 1 Anahit Ghochikyan Richard Cadagan Dmitriy Zamarin Irina Petrushina Nina Movsesyan Luis Martinez-Sobrido4 Randy A Albrecht3 5 Adolfo García-Sastre3 5 6 and Michael G Agadjanyan1 2 Abstract Background Numerous pre-clinical studies and clinical trials demonstrated that induction of antibodies to the p-amyloid peptide of 42 residues Ap42 elicits therapeutic effects in Alzheimer s disease AD . However an active vaccination strategy based on full length Ap42 is currently hampered by elicitation of T cell pathological autoreactivity. We attempt to improve vaccine efficacy by creating a novel chimeric flu vaccine expressing the small immunodominant B cell epitope of Ap42. We hypothesized that in elderly people with pre-existing memory Th cells specific to influenza this dual vaccine will simultaneously boost anti-influenza immunity and induce production of therapeutically active anti-Ap antibodies. Methods Plasmid-based reverse genetics system was used for the rescue of recombinant influenza virus containing immunodominant B cell epitopes of Ap42 Ap1-7 10 . Results Two chimeric flu viruses expressing either 7 or 10 aa of Ap42 flu-Ap1-7 or flu-Ap1-10 were generated and tested in mice as conventional inactivated vaccines. We demonstrated that this dual vaccine induced therapeutically potent anti-Ap antibodies and anti-influenza antibodies in mice. Conclusion We suggest that this strategy might be beneficial for treatment of AD patients as well as for prevention of development of AD pathology in pre-symptomatic individuals while concurrently boosting immunity against influenza. Introduction Alzheimer s disease AD is the most common form of dementia in the elderly