tailieunhanh - báo cáo hóa học:" Inhibition of human immunodeficiency virus type-1 (HIV-1) glycoprotein-mediated cell-cell fusion by immunor (IM28)"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Inhibition of human immunodeficiency virus type-1 (HIV-1) glycoprotein-mediated cell-cell fusion by immunor (IM28) | Virology Journal BioMed Central Research Open Access Inhibition of human immunodeficiency virus type-1 HIV-I glycoprotein-mediated cell-cell fusion by immunor IM28 Donatien Mavoungou 1 Virginie Poaty-Mavoungou1 2 Marie- Yvonne Akoume3 Brice Ongali4 and Elie Mavoungou1 2 Address 1Centre de recherche sur les pathologies hormonales Libreville Gabon 2Department of Parasitology Institute for Tropical Medicine University of Tubingen Tubingen Germany 3Département de Pharmacologie Université de Montreal Montreal Quebec Canada and 4Département de Physiologie Université de Montreal Montreal Quebec Canada Email Donatien Mavoungou - crph2000@ Virginie Poaty-Mavoungou - virpoaty@ Marie- Yvonne Akoume - crph2000@ Brice Ongali - Elie Mavoungou - Corresponding author Published II February 2005 Received 22 December 2004 Accepted 11 February 2005 Virology Journal 2005 2 9 doi 1743-422X-2-9 This article is available from http content 2 1 9 2005 Mavoungou et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Immunor IM28 an analog of dehydroepiandrosterone DHEA inhibits human immunodeficiency virus type-1 HIV-1 by inhibiting reverse transcriptase. We assessed the ability of IM28 to inhibit the cell-cell fusion mediated by HIV envelope glycoprotein in an in vitro system. For this purpose we co-cultured a T-cell line expressing stably HIV-1 glycoprotein envelopes with an equal number of 293 CD4 another T cell line expressing CD4 and with the SupT1 cell line with or without IM28. Results In the absence of IM28 fused with 293 CD4 inducing numerous large syncytia. Syncytia appeared more rapidly when .