tailieunhanh - Báo cáo y học: "Cell Cycle Arrest by a Natural Product via G2/M Checkpoint"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Cell Cycle Arrest by a Natural Product via G2/M Checkpoint. | Int. J. Med. Sci. 2005 2 64 International Journal of Medical Sciences Research paper ISSN 1449-1907 2005 2 2 64-69 2005 Ivyspring International Publisher. All rights reserved Cell Cycle Arrest by a Natural Product via G2 M Checkpoint Received Accepted Published Sharon Chui-Wah Luk Stephanie Wing-Fai Siu Chun-Kit Lai Ying-Jye Wu Shiu-Fun Pang Technology Development CK Life Sciences Int l Inc. 2 Dai Fu Street Tai Po Industrial Estate Hong Kong China Abstract CKBM is a natural product that exhibits a novel anti-tumor activity through the induction of cell cyCle arrest and apoptosis. We have investigated its effects on cell cycle regulation using a gastric cancer cell line AGS. The effects of CKBM on cell proliferation cell cycle regulation and apoptosis were analyzed using BrdU 5-bromo-2 -deoxyuridine cell proliferation assay and flow cytometric analysis respectively. Specific cellular protein expressions were measured using Western blot analysis. Flow cytometric analysis indicated that CKBM induced G2 M cell cycle arrest and apoptosis whereas differential protein expressions of p21 p53 and 14-3-3Ơ stratifin using Western blot analysis were enhanced. The differential expressions of p21 p53 and 14-3-3Ơ in AGS cancer cells after CKBM treatment may play critical roles in the G2 M cell cycle arrest that blocks cell proliferation and induces apoptosis. Key words 14-3-3Ơ stratifin G2 M arrest cell proliferation checkpoint protein Author biography Sharon Chui-Wah Luk . is Project Manager of CK Life Sciences Int l Inc. She graduated from University of Alberta with a bachelor degree before completing . study in the Department of Biochemistry The Chinese University of Hong Kong. Her research interest is in cancer therapy. Stephanie Wing-Fai Siu M. Phil. is Science Officer of CK Life Sciences Int l Inc. She graduated from University of Hong Kong in the Department of Physiology. Her current research is in anti-cancer .