tailieunhanh - báo cáo hóa học:" Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections | Virology Journal BioMed Central Research Open Access Reference gene selection for quantitative real-time PCR analysis in virus infected cells SARS corona virus Yellow fever virus Human Herpesvirus-6 Camelpox virus and Cytomegalovirus infections Aleksandar Radonic 1 Stefanie Thulke1 Hi-Gung Bae2 Marcel A Muller2 Wolfgang Siegert1 and Andreas Nitsche2 Address 1Charité - CCM Medizinische Klinik II . Hamatologie Onkologie Berlin Germany and 2Robert Koch Institut ZBS 1 Berlin Germany Email Aleksandar Radonic - Stefanie Thulke - Hi-Gung Bae - baeh@ Marcel A Muller - muellerm@ Wolfgang Siegert - Andreas Nitsche - nitschea@ Corresponding author Published 10 February 2005 Received 03 February 2005 Accepted 10 February 2005 Virology Journal 2005 2 7 doi I743-422X-2-7 This article is available from http content 2 1 7 2005 Radonic et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus Human Herpesvirus-6 Camelpox virus SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However over all P-actin is an unsuitable as reference gene whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells. Background .