tailieunhanh - o cáo hóa học:" Multiplex Zymography Captures Stage-specific Activity Profiles of Cathepsins K, L, and S in Human Breast, Lung, and Cervical Cancer"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Multiplex Zymography Captures Stage-specific Activity Profiles of Cathepsins K, L, and S in Human Breast, Lung, and Cervical Cancer | JOURNAL OF TRANSLATIONAL MEDICINE Multiplex Zymography Captures Stage-specific Activity Profiles of Cathepsins K L and S in Human Breast Lung and Cervical Cancer Chen and Platt Chen and Platt Journal ofTranslational Medicine 2011 9 109 http content 9 1 109 14 July 2011 2 BioMed Central Chen and Platt Journal of Translational Medicine 2011 9 109 http content 9 1 109 RESEARCH JOURNAL OF TRANSLATIONAL MEDICINE Open Access Multiplex Zymography Captures Stage-specific Activity Profiles of Cathepsins K L and S in Human Breast Lung and Cervical Cancer Binbin Chen and Manu O Platt Abstract Background Cathepsins K L and S are cysteine proteases upregulated in cancer and proteolyze extracellular matrix to facilitate metastasis but difficulty distinguishing specific cathepsin activity in complex tissue extracts confounds scientific studies and employing them for use in clinical diagnoses. Here we have developed multiplex cathepsin zymography to profile cathepsins K L and S activity in 10 pg human breast lung and cervical tumors by exploiting unique electrophoretic mobility and renaturation properties. Methods Frozen breast lung and cervix cancer tissue lysates and normal organ tissue lysates from the same human patients were obtained 28 breast tissues 23 lung tissues and 23 cervix tissues minced and homogenized prior to loading for cathepsin gelatin zymography to determine enzymatic activity. Results Cleared bands of cathepsin activity were identified and validated in tumor extracts and detected organ-and stage-specific differences in activity. Cathepsin K was unique compared to cathepsins L and S. It was significantly higher for all cancers even at the earliest stage tested stage I for lung and cervix n 6 p .05 and stage II for breast n 6 p .0001 . Interestingly cervical and breast tumor cathepsin activity was highest at the earliest stage we tested stages I and II respectively and then were significantly lower at