tailieunhanh - o cáo hóa học:" Effects of transplantation with bone marrowderived mesenchymal stem cells modified by Survivin on experimental stroke in rats"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Effects of transplantation with bone marrowderived mesenchymal stem cells modified by Survivin on experimental stroke in rats | Liu et al. Journal of Translational Medicine 2011 9 105 http content 9 1 105 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Effects of transplantation with bone marrow-derived mesenchymal stem cells modified by Survivin on experimental stroke in rats 1 3 4 1 1 1 1 Nan Liu Yixian Zhang Lin Fan Mingzhou Yuan Houwei Du Ronghua Cheng Deshan Liu and Feifei Lin Abstract Background This study was performed to determine whether injury induced by cerebral ischemia could be further improved by transplantation with bone marrow-derived mesenchymal stem cells MSCs modified by Survivin SVV . Methods MSCs derived from bone marrow of male Sprague-Dawley rats were infected by the self-inactive lentiviral vector GCFU carrying green fluorescent protein GFP gene and SVV recombinant vector GCFU-SVV . In vitro vascular endothelial growth factor VEGF and basic fibroblast growth factor bFGF were detected in infected MSCs supernatants under hypoxic conditions by ELSIA. In vivo experiments consisted of three groups one receiving intravenous injection of 500 gl of phosphate-buffered saline PBS without cells control group and two groups administered the same volume solution with either three million GFP-MSCs group GFP or SVV GFP-MSCs group SVV . All animals were submitted to 2-hour middle cerebral artery occlusion MCAO and then reperfusion. Differentiation and survival of the transplanted MSCs were determined by confocal microscope. Western blot was used to detect the expression of VEGF and bFGF in ischemic tissue. A 2 3 5-triphenyltetrazolium chloride TTC staining was used to assess the infarct volume. Evaluation of neurological function was performed using a modified Neurological Severity Score mNSS . Results In vitro modification with SVV further increased secretion of VEGF and bFGF under hypoxic condition. In vivo only very few transplantated cells co-expressed GFP and NeuN. The survival transplanted cells in the group SVV was at 4 days